Molecular Detection of the Mutations in Khoderi Olive cultivar
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https://doi.org/10.14295/cs.v17.4380Resumo
The characterization of olive cultivars using molecular markers is necessary for genetic diversity conservation, cultivar verifiction, and the identification of locally adapted genotypes. This study searches for genetic variation in the Khoderi olive cultivar from Karbala, Iraq, utilization two genetic molecular regions: the nuclear ribosomal ITS1 spacer and the plastid matK gene. Through DNA was extracted using a modified CTAB protocol, amplified by PCR, and get sequenced utilization Sanger sequencing. Alignment and comparative analysis with reference sequences deposited in Gen-Bank the results revealed 92.3% similarity of the ITS1 sequence with the Khoderi-Bakraju cultivar with serial number (OQ134700.1), indicating genetic affinity but insufficient similarity for confirmed cultivar identity. The matK region showed 96% similarity with an Olea europaea subsp. europaea plastid genome with serial number (MG372119.1), we notice reflecting high conservation of the chloroplast coding sequences. Multiple SNPs and small indels were detected in both regions, representing intraspecific polymorphisms. The combined data suggest that the studied sample belongs to the broader O. europaea gene pool but may represent a distinct local genotype. Further analyses using high-resolution molecular markers (SSR, SNP-based GBS) are recommended to refine cultivar differentiation.
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Copyright (c) 2026 Ziena Thamer A. Al Rufaye, Ayam Razak Abdal Katham, Hassan Jameel Jawad
Este trabalho está licenciado sob uma licença Creative Commons Attribution-NonCommercial 4.0 International License.
A revista se reserva o direito de efetuar, nos originais, alterações de ordem normativa, ortográfica e gramatical, com vistas a manter o padrão culto da língua, respeitando, porém, o estilo dos autores. As provas finais serão enviadas aos autores. Os trabalhos publicados passam a ser propriedade da revista Comunicata Scientiae, ficando sua reimpressão total ou parcial, sujeita à autorização expressa da direção do periódico. Deve ser consignada a fonte de publicação original. Os originais não serão devolvidos aos autores. As opiniões emitidas pelos autores dos artigos são de sua exclusiva responsabilidade.
