Morphological characterization of vaginal epithelial cells of santa inês ewes subjected to estrus synchronization

  • Camila Vasconcelos Ribeiro Universidade Federal do Piauí
  • Tábatta Arrivabene Neves Universidade Federal do Piauí
  • Glaucia Brandão Fagundes Programa de Pós Graduação em Zootecnia, UFPI
  • Dayana Maria do Nascimento Universidade Federal do Piauí, programa multiprofissional em saúde, área de reprodução animal
  • Cleidson Manoel Gomes da Silva Universidade Federal do Piauí, campus Professora Cinobelina Elvas, Bom Jesus-PI
  • Mônica Arrivabene Universidade Federal do Piauí
  • Francisca Elda Ferreira Dias Universidade Federal do Tocantins, Escola de Medicina Veterinária e Zootecnia, campus de Araguaína
  • Tânia Vasconcelos Cavalcante Universidade Federal do Piauí, campus de Teresina

Abstract

Vaginal cytology analysis has been used to evaluate the different stages of estrous cycle of several species; it presents a direct correlation with the animal’s hormonal state and provides essential information about the female reproductive tract conditions. Two staining methods were tested to evaluate the vaginal epithelial cell morphology of nulliparous and multiparous ewes during the estrus period. An intravaginal device impregnated with medroxyprogesterone acetate was kept into 10 nulliparous and 10 multiparous ewes for 14 days for estrus synchronization. Then, the progesterone device was withdrawn, and 300 IU of eCG was administered intramuscularly. Vaginal smears were prepared for posterior staining with Panotico or Giemsa stains when estrus was detected. The cells were classified into nucleated superficial, anucleate superficial, intermediate, parabasal, and basal. The Panotico and Giemsa staining of the different cell types studied were satisfactory. A predominance of intermediate epithelial cells (p<0.05) was found after staining. No difference in percentages of the different types of vaginal epithelial cells between nulliparous and multiparous ewes were found. Therefore, both staining methods were efficient, and a predominance of intermediate cells is found in nulliparous and multiparous ewes during the estrus period.

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Author Biographies

Camila Vasconcelos Ribeiro, Universidade Federal do Piauí
Acadêmica de Medicina Veterinária
Tábatta Arrivabene Neves, Universidade Federal do Piauí
Acadêmica de Medicina Veterinária
Glaucia Brandão Fagundes, Programa de Pós Graduação em Zootecnia, UFPI
Médica Veterinária, Mestranda em Zootecnia, Reprodução animal
Dayana Maria do Nascimento, Universidade Federal do Piauí, programa multiprofissional em saúde, área de reprodução animal
Médica Veterinária pela UFPI / CPCE, campus de Bom Jesus, Residente em Programa Multiprofissional em saúde na área de reprodução animal
Cleidson Manoel Gomes da Silva, Universidade Federal do Piauí, campus Professora Cinobelina Elvas, Bom Jesus-PI
Professora Adjunto do Curso de Medicina Veterinária / UFPI / CPCE, na área de Morfologia Veterinária
Mônica Arrivabene, Universidade Federal do Piauí
Professora Associada do Departamento de Clínica e Cirurgia Veterinária / UFPI, área de Clínica Médica de Equinos e Obsterícia Veterinária
Francisca Elda Ferreira Dias, Universidade Federal do Tocantins, Escola de Medicina Veterinária e Zootecnia, campus de Araguaína
Professora Associada da EMVZ / UFT, área de Reprodução Animal
Tânia Vasconcelos Cavalcante, Universidade Federal do Piauí, campus de Teresina
Departamento de Clínica e Cirurgia Veterinária (DCCV) Reprodução Animal

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Published
17-04-2019
How to Cite
Ribeiro, C., Neves, T., Fagundes, G., do Nascimento, D. M., da Silva, C. M., Arrivabene, M., Dias, F. E., & Cavalcante, T. (2019). Morphological characterization of vaginal epithelial cells of santa inês ewes subjected to estrus synchronization. Comunicata Scientiae, 10(1), 5-9. https://doi.org/10.14295/cs.v10i1.2756
Section
Original Article