Vegetative propagation of Juniperus chinensis

Rosimeri de Oliveira Fragoso, Katia Christina Zuffellato-Ribas, Gustavo Macanhão, Carlos André Stuepp, Henrique Soares Koehler


Juniperus chinensis L. var. kaizuka, belonging to the Cupressaceae family and known as kaizuka is a species widely used in landscaping, being different from the other varieties of the species mainly because of its tortuous growth habits. The sexual reproduction of Juniperus chinensis has disadvantages and its propagation by cuttings can facilitate the production of seedlings. Thus, this study aimed to evaluate the effect of three different concentrations of the synthetic auxin indolebutyric acid (0, 1500 and 3000 mg L-1) to promote rooting of Juniperus chinensis var. kaizuka, collected in the basal (0.5 to 2.0 m), middle (2.1 to 3.5 m) and apical (3.6 to 5.0 m) portions of the stock plant, during winter, spring and autumn of 2012, with the intent to subsidize the development of a propagation protocol for the species. After 140 days in the greenhouse, it was observed that choosing the proper season to collect cuttings is essential for the viability of vegetative propagation technique for Juniperus chinensis var. kaizuka, with the recommendation of spring as the more suitable season for rooting development. However, although the application of AIB and the collection height in the stock plant have shown significant effects on some variables, it wasn’t observed a standard, related to these factors, producing a greater induction of the radicial system of the species.


auxin, rooting, season, gradient of juvenility, cone of juvenility


Abu-Zahra, T.R., Al-Shadaideh, A.N., Abubaker, S.M., Qrunileh, I.M. 2013. Influence of auxin concentrations on different ornamental plants rooting. International Journal of Botany 9: 96-99.

Adams, R.P. 2014. Junipers of the world: the genus Juniperus. Trafford Publishing, Bloomington, USA. 422 p.

Ahani, H., Jalilvand, H., Nasr, S.M.H., Kouhbanani, H.S., Ghazi, M.R., Mohammadzadeh, H. 2013. Reproduction of juniper (Juniperus polycarpos) in Khorasan Razavi, Iran. Forest Science and Practice 15: 231-237.

Alcantara, G.B., Ribas, L.L.F., Higa, A.R., Zuffellato-Ribas, K.C. 2008. Efeitos do ácido indolbutírico (AIB) e da coleta de brotações em diferentes estações do ano no enraizamento de mini-estacas de Pinus taeda L. Scientia Forestalis 36:151-156.

Dias, J.P.T., Ono, E.O., Rodrigues, J.D. 2011. IBA e carboidratos no enraizamento de brotações procedentes de estacas radiciais de Rubus spp. Revista Brasileira de Fruticultura 3: 666-671.

Durbak, A., Yao, H., Mcsteen, P. 2012. Hormone signaling in plant development. Current opinion in plant biology 15: 92-96.

Esmaeilnia, M., Jalali, S.GH.A., Tabari, M., Hosseini, S.M. 2006. Influence of plant growth regulator IBA on vegetative propagation of Juniperus excelsa. Iranian Journal of Forest and Poplar Research 14: 221–227.

Ferriani, A.P., Bortolini, M.F., Zuffellato-Ribas, K.C., Koehler, H.S. 2006. Propagação vegetativa de estaquia de azaléia arbórea (Rhododendron Thomsonii HOOK. f.). Semina, 27: 35-42.

Ferriani, A., Borges, M., Zuffellat0-Ribas, K.C., Carpanezzi, A., Koehler, H. 2007. Influência da época do ano e das diferentes formas de aplicação de ácido naftaleno acético (ANA) no enraizamento de Mikania micrantha Kunth. Revista Brasileira de Plantas Medicinais 9: 102-107.

Gorman, J. Propagation of Juniperus chinensis torulosa. 1961. Book Proceedings 11th annu. Mtg Plant Prop. Soc., Califórnia, USA.

Hartmann, H.T., Kester, D.E., Davies JR, F.T., Geneve, R.L. 2011. Hartmann & Kerster´s plant propagation: principles and practices. Prentice Hall, New Jersey, USA. 915 p.

Henrique, A., Campinhos, E.N., Ono, E.O., Pinho, S.Z.D. 2006. Effect of plant growth regulators in the rooting of Pinus cuttings. Brazilian Archives of Biology and Technology 49:189-196.

Ju, J.B., Kim, J.S., Choi, C.W., Lee, H.K., Oh, T.K., Kim, S.C. 2008. Comparison between ethanolic and aqueous extracts from Chinese juniper berries for hypoglycaemic and hypolipidemic effects in alloxan-induced diabetic rats. Journal of ethnopharmacology 115:110-115.

Kebede, M., Hulten, H., Balcha, G. 2013. Vegetative propagation of juvenile leafy stem cuttings of Prunus africana (Hook.f.) Kalkm and Syzygium guineense (Willd.) DC. International Journal of Botany 9: 30-36.

khoushnevis, M., Ali Ahmad Koruri, S., Teymouri, M., Matinizadeh, M., Rahmani, A., Shirvani, A. 2008. The effect of different treatments on rooting of Juniperus excels cutting. Iranian Journal of Forest and Poplar Research 16:158-167.

Kibbler, H., Johnston, M.E., Williams, R.R. 2004. Adventitious root formation in cuttings of Backhousia citriodora F. Muell: 2. Seasonal influences of temperature, rainfall, flowering and auxins on the stock plant. Scientia horticulturae 102: 343-358.

Kim, S.J., Jung, J.Y., Kim, H.W., Park, T. 2008. Anti-obesity effects of Juniperus chinensis extract are associated with increased AMP-activated protein kinase expression and phosphorylation in the visceral adipose tissue of rats. Biological and Pharmaceutical Bulletin 31: 1415-1421.

Lawson, T., Kramer, D.M., Raines, C.A. 2012. Improving yield by exploiting mechanisms underlying natural variation of photosynthesis. Current Opinion in Biotechnology 23: 215-220.

Lorenzi, H. 2003. Árvores exóticas no Brasil: madeireiras, ornamentais e aromáticas. Instituto Plantarum, Nova Odessa, Brasil. 368 p.

Negash, L. 2002. Successful vegetative propagation techniques for the threatened African pencil cedar. Forest ecology and management 161: 53-64.

Oliveira, M.C., Ribeiro, J.F. 2013. Enraizamento de estacas de Euplassa inaequalis (Pohl) Engl. de mata de galeria em diferentes estações do ano. Bioscience Journal 29: 991-999.

Osterc, G., Štampar, F. 2011. Differences in endo/exogenous auxin profile in cuttings of different physiological ages. Journal of plant physiology 168: 2088-2092.

Passardi, F., Cosio, C., Penel, C., Dunand, C. 2005.

Peroxidases have more functions than a Swiss army knife. Plant Cell Reports 24: 255-265.

Pop, T.I., Pamfil, D., Bellini, C. 2011. Auxin Control in the Formation of Adventitious Roots. Notulae Botanicae. Horti Agrobotanici Cluj-Napoca 39: 307-316.

Pio, R., Ramos, J.D., Chalfun, N.N.J., Gontijo, T.C.A., Mendonça, V., Carrijo, E.P., Chagas, E.A. 2006. Propagação de estacas apicais de figueira: diferentes ambientes, ácido indolbutírico e tipo de estaca. Ciência e Agrotecnologia 30: 1021-1026.

Radomski, M.I., Wisniewski, C., Curcio, G.R., Rachwal, M.G., Santos, C.A.M. 2004. Caracterização de ambientes de ocorrência natural e sua influência sobre o peso específico e o teor de polifenóis totais de folhas de espinheira-santa (Maytenus ilicifolia Mart.). Revista Brasileira de Plantas Medicinais 6: 36-43.

Stuepp, C.A., Pereira, G.P., Zem, L.M., Peña, M.L.P., Bueno, P. M.C., Spader, V., Zuffellato-Ribas, K.C., Rosa, G.M. 2013. Enraizamento de melaleuca: influência da altura de coleta das estacas e aplicação de iba. Colloquium Agrariae 9:01-09.

Stuepp, C.A., Zuffellato-Ribas, K.C., Macanhão, G., Fragoso, R.O., Rickli, H.C. 2014. Estaquia caulinar de Juniperus chinensis var. kaizuka: concentrações de IBA e diferentes alturas de coleta. Agrarian 7.

Taiz, L., Zeiger, E. 2009. Fisiologia vegetal. Artmed, Porto Alegre, Brasil. 819 p.

Valdés, A.E., Fernández, B., Centeno, M.L. 2004. Hormonal changes throughout maturation and ageing in Pinus pinea. Plant Physiology and Biochemistry 42: 335-340.

Vignolo, G.K., Picolotto, L., Gonçalves, M.A., Pereira, I.D.S., Antunes, L.E.C. 2014. Presença de folhas no enraizamento de estacas de amoreira-preta. Ciência Rural 44: 467-472.

Tehranifar, A., Tabar, S.M., Selahvarzi, Y., Balandary, A., Kharrazi, M. 2014. Biochemical changes in barberries during adventitious root formation: the role of indole-3-butyric acid and hydrogen peroxide. Spanish Journal of Agricultural Research 12: 477-485.

Wendling, I., Trueman, S.J., Xavier, A. 2014a. Maturation and related aspects in clonal forestry—Part I: concepts, regulation and consequences of phase change. New Forest, p. 1-23.

Wendling, I., Trueman, S.J., Xavier, A. 2014b. Maturation and related aspects in clonal forestry—part II: reinvigoration, rejuvenation and juvenility maintenance. New Forests, p. 1-14.


Copyright (c) 2016 Rosimeri de Oliveira Fragoso, Katia Christina Zuffellato-Ribas, Gustavo Macanhão, Carlos André Stuepp, Henrique Soares Koehler

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.