In vitro root development under different IBA concentrations and culture media

Abstract

In vitro root cultivation is a method that provides high production and volume of roots quickly due to controlled environmental conditions. However, few studies report the in vitro development of excised roots for the most diverse plant species. The objective was to evaluate the in vitro development of excised mint (Menta piperita L.) and tomato (Solanum lycopersicum L.) roots under different concentrations of IBA and verify tomato roots' growth in different cultivation media. Segments of 2 cm of roots were incubated in DSD1 culture medium with concentrations of 0, 0.25, 0.5, 1.0, and 2.0 mg. L^-1 of AIB. Afterward, the 'M,' 'MSR', and DSD1 media were tested, supplemented with 1 mg L^-1 of IBA. The cultivation of tomato roots shows greater in vitro development than mint, and 1 mg L^-1 of IBA concentration was the most effective for tomatoes in DSD1 medium. The MSR medium resulted in a higher number and length of roots. Applying IBA increases the in vitro development of excised tomato roots compared to mint. For tomatoes, the concentration of 1 mg L^-1 of IBA stimulates the most significant development of the roots. The MSR medium has the highest development of tomato roots at 60 days after in vitro introduction.