"Biosynthesis of silver nanoparticles from Staphylococcus lentus isolated from Ocimum basilicum and their antibacterial activity"

Abstract

The present study was isolated and identification of endophytic bacteriumisolated from Ocimum basilicum by surface sterilization method. Bacterial strain was identifiedas Sphylococcus lentus.Bacterium isolate was used to detect their ability toprepare silver nanoparticles. The results showed change in color silver nitrate solution 1mM brown, studied the spectrum of absorption of UV, visible spectroscopy of the solution silver particles nano second stepin making sure the formation of particles and found to be located on the wavelength of 400 nm to bacteriaOcimum basilicumalso show that the X-ray diffraction peak was at (103) and at the angle (34.1). Additinary the results showed clear images in the scanning electron microscope size and shape, Size ranged between20.4 -93.04nm.The results revealed thatbAgNPs synthesized from Sphylococcus lentus. have antibacterial activity against Gram-negativeEscherichia coli, Gram-positive Staphylococcus aureus