Extracts of Tagetes minuta L. front of bacteria regarding bovine mastitis

  • Gabriela Berguenmaier de Olanda Universidade Federal de Pelotas
  • Gilberto Antônio Peripolli Bevilaqua EMBRAPA Clima Temperado
  • Luiz Filipe Damé Schuch Universidade Federal de Pelotas
  • Luciana Souza Prestes Universidade Federal de Pelotas
  • Ricardo Batista Job Universidade Federal de Pelotas

Abstract

Bovine mastitis is a problem in the context of animal health. However medicinal plants are a management strategy in agroecological production systems. In this context, the plant Tagetes minuta L. plays a leading role, due to its recognition in the popular pharmacopeia. Thus, the objective of this work was to assess the effect of hydroalcoholic extracts of T. minuta from different collection sites compared to ten bacteria related to bovine mastitis. The antimicrobial evaluation was done through the microdilution technique in syrup, in 96 well microplates in triplicate, for the determination of Minimal Bacterial Concentration (MBC) (%). In the microplates, the extracts of T. minuta and the inoculums of the bacteria were placed in addition to the growth control of the bacteria and the control of the extracts, which were incubated for 72 hours at 37°C in an oven. After this time, aliquots of 5μL of each orifice were transferred to plates containing 5% equine defibrinated blood agar and maintained in incubation for 24 hours at the same previous temperature. With this, the growth readings were performed, with the subsequent interpretation of the results by the geometric mean of the MBC (%). The results indicate that there is a difference in sensitivity to microorganisms by the extracts as a function of the collection sites and both present antimicrobial action.

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References

Chamorro, E.R. Ballerini, G., Sequeira, A.F., Velasco, G.A., Zalazar, M.F. 2008. Chemical composition of essential oil from Tagetes minuta L. leaves and flowers. Journal of the Argentine Chemical Society 96: p.80-86.

Cornelius, W.W., Wycliffe, W. 2016. Tagetes (Tagetes minuta) oils. In: PREEDY, V.R. (ed.). Essential Oils in Food Preservation, Flavor and Safety. Academic Press, p. 791–802.

Gakuubi, M.M., Wanzala, W., Wagacha, J.M., Dossaji, S.F. 2016. Bioactive properties of Tagetes minuta L. (Asteraceae) essential oils: A review. American Journal of Essential Oils and Natural Products 4: 27-36.

Gonçalves, C.L., Schiavon, D.B.A., Mota, F.V., Faccin, A., Schubert, R.N., Schiedeck, G., Schuch, L.F.D. 2013. Actividad antibacteriana de los extractos de Cymbopogon citratus, Elionurus sp. y Tagetes minuta contra bactérias que causan mastites. Revista Cubana de Plantas Medicinales 18: 487-494.

López, M.L., Bonzani, N.E., Zygadlo, J.A. 2009. Allelopathic potential of Tagetes minuta terpenes by a chemical, anatomical and phytotoxic approach. Biochemical Systematics and Ecology 36: 882-890.

Lovatto, P.B., Mauch, C.R., Schiedeck, G. 2011. Bioatividade de extratos aquosos de Tagetes minuta (Asteraceae) sobre Brevicoryne brassicae (L., 1758) (Hemiptera: Aphididae) e viabilidade de sua utilização no manejo agroecológico de hortaliças. Cadernos de Agroecologia 6: 1-5.

Marinho, M.L., Alves, M.S., Rodrigues, M.L.C., Rotondano, T.E.F., Vidal, I.F., Silva, W.W., Athayde, A.C.R. 2007. A utilização de plantas medicinais em medicina veterinária: um resgate do saber popular. Revista Brasileira de Plantas Medicinais 9: 64-69.

Mota, F.V., Schuch, L.F D., Gonçalves, C.L., Faccin, A., Schiavon, D.B.A., Bohm, B.C., Lessa, L.F. 2013. Actividad antibacteriana de los extractos de Syzygium cumini (L.) Skeels (jambolán) frente a los microorganismos asociados a la mastitis bovina. Revista Cubana de Plantas Medicinales 18: 495-501.

Oyarzabal, M.E.B., Schuch, L.F.D., Prestes, L.S., Schiavon D.B.A., Rodrigues, M.R.A., Mello, J.R.B. 2011. Actividad antimicrobiana de aceite esencial de Origanum vulgare L. ante bacterias aisladas en leche de bovino. Revista Cubana de Plantas Medicinales 16: 0-0.

Peres Neto, F., Zappa, V. 2011. Mastite em vacas leiteiras - revisão de literatura. Revista Científica Eletrônica de Medicina Veterinária 9: 1-28.

Prestes, L.S., Frascolla, R., Santin, R., Santos, M.A.Z., Schram, R.C., Rodrigues, M.R.A., Schuch, L.F.D., Meireles, M.C.A. 2008. Actividad de extractos de orégano y tomillo frente a microorganismos associados con otitis externa. Revista Cubana de Plantas Medicinales 13: 0-0.

Rachuonyo, H.O., Ogola, P.E., Arika, W.M., Wambani, J.R., Gatheri, G.W., NYAMACHE, A.K. 2016. Combined effect of crude leaf extracts of selected medicinal plants against selected enteric bacterial pathogens and Candida albicans. Journal of Antimicrobial Agents 2: 1-5.

Ramakrishna A., Gokare, A.R. 2011. Influence of abiotic stress signals on secondary metabolites in plants. Plant Signaling & Behavior 6: 1720-1731.

Shahzadi, I., Shah, M.M. 2015. Acylated flavonol glycosides from T. minuta with antibacterial activity. Fronties in Pharmacology 6: 1-10.

Schuch, L., Wiest, J., Coimbra, H., Prestes, L., Toni, L., Lemos, J. 2008. Cinética da Atividade Antibacteriana in vitro de Extratos Naturais frente a microrganismos relacionados à mastite bovina. Ciência Animal Brasileira 9: 161-169.

Tereschuk, M.L., Riera, M.V.Q., Castro, G.R., Abdalla, L.R. 1997. Antimicrobial activity of flavonoids from leaves of Tagetes minuta. Journal of Etnopharmacology 56: 227-232.

Wanzala, W., Takken, W., Mukabana, W.R., Pala, A.O., Hassanali, A. 2012. Ethnoknowledge of Bukusu community on livestock tick prevention and control in Bungoma district, western Kenya. Journal of Ethnopharmacology 140: 298–324.

Published
17-04-2019
How to Cite
Berguenmaier de Olanda, G., Peripolli Bevilaqua, G., Damé Schuch, L., Souza Prestes, L., & Batista Job, R. (2019). Extracts of Tagetes minuta L. front of bacteria regarding bovine mastitis. Comunicata Scientiae, 10(1), 1-4. https://doi.org/10.14295/cs.v10i1.2623
Section
Original Article