Molecular identification of actinomycetes with antimicrobial, antioxidant and anticancer properties

  • Mohamed S Abdel-Aziz National Research Center
  • Amal S Hathout National Research Center
  • Aziza A El-Neleety National Research Center
  • Ahmed A Hamed National Research Center
  • Bassem A Sabry National Research Center
  • Soher E Aly National Research Center
  • Mosaad A Abdel-Wahhab National Research Center

Abstract

The objectives of this study were to isolate and identify the actinomycetes strains from the soil and marine sediments and to evaluate the antimicrobial, antioxidant and cytotoxic activity of their bioactive secondary metabolites. Eight actinomycetes strains were isolated from soil and marine sediment samples collected from different areas in Egypt. Only three actinomycetes exhibited a wide spectrum of antimicrobial activities. They were active in vitro against microbial pathogen viz: Staphylococcus aureus, Escherichia coli, Salmonella typhi, Aspergillus parasiticus, Fusarium solani and Fusarium oxysporum. These promising isolates were selected and identified using molecular identification technique and identified as Streptomyces spp. The crude extracts from the three Streptomyces exhibited potent antimicrobial activities against a set of microbial pathogens as well as antioxidant and anticancer activity in human hepatocellular carcinoma cell line (HepG2). The crude extract of Streptomyces isolate D showed antitumor activity with lC50 0.85 µg/ mL. Forty compounds were identified from the two most promising ethyl acetate extracts of culture broth of Streptomyces sp. (D-EGY) by Gas Chromatography-Mass Spectrometry analysis. It could be concluded that the streptomycetes isolated from the Egyptian environment are promising candidates as antimicrobial, antioxidant and anticancer.

Downloads

Download data is not yet available.

Author Biographies

Mohamed S Abdel-Aziz, National Research Center
Microbial Chemistry Department
Amal S Hathout, National Research Center
Food Toxicology & Contaminants Department
Aziza A El-Neleety, National Research Center
Food Toxicology & Contaminants Department
Ahmed A Hamed, National Research Center
Microbial Chemistry Department
Bassem A Sabry, National Research Center
Food Toxicology & Contaminants Department
Soher E Aly, National Research Center
Food Toxicology & Contaminants Department
Mosaad A Abdel-Wahhab, National Research Center
Food Toxicology & Contaminants Department

References

Abdalhai, M.H., Fernandes, A.M., Xia, X., Musa, A., Ji, J., Sun, X. 2015. Electrochemical Genosensor To Detect Pathogenic Bacteria (Escherichia coli O157:H7) As Applied in Real Food Samples (Fresh Beef) To Improve Food Safety and Quality Control. Journal of Agriculture and Food Chemistry 63(20):5017-5025.

Abdel-Aziz, M.S., Ibrahim, N.A., Eid, B.M. 2014. Biosynthesized silver nanoparticles for antibacterial treatment of cellulosic fabrics using O2-plasma. AATCC Journal Research 1(1): 6-12.

Abdelfattah, M.S., Elmallah, M.I.Y., Hawas, U.W., Abou El-Kassema, L.T., Eid, M.A.G. 2016. Isolation and characterization of marine-derived actinomycetes with cytotoxic activity from the Red Sea coast. Asian Pacific Journal of Tropical Biomedicine 6(8):651-657.

Abd-Elnaby, H., Abo-Elala, G., Abdel-Raouf, U., Abdelwahab, A., Hamed, M. 2016. Antibacterial and anticancer activity of marine Streptomyces parvus: optimization and application. Biotechnology and Biotechnological Equipment 30(1): 180-191.

Alam, M.K., Ahmed, S., Anjum, S., Akram, M., Shah, S.M., Wariss, H.M., Usmanghani, K. 2016. Evaluation of antipyretic activity of some medicinal plants from Cholistan desert Pakistan. Pakistan Journal of Pharmaceutical Sciences 29(2):529-533.

Antoszczak, M., Maj, E., Napiórkowska, A., Stefańska, J., Augustynowicz-Kopeć, E., Wietrzyk, J., Janczak, J., Brzezinski, B., Huczyński, A. 2014. Synthesis, anticancer and antibacterial activity of salinomycin N-benzyl amides. Molecules 19(12): 19435-59.

Arunachalam, R., Wesely, E.G., George, J., Annadurai, G. 2010. Novel approaches for identification of Streptomyces noboritoensis TBG-V20. Current Research in Bacteriology 3(1): 15-26.

Ashok, G., Karthikeyan, P., Panneerselvam, A., Senthilkumar G. 2014. Effect of antimicrobial activity of UV mutated actinomycetes SP isolated from mangroves. European Journal of Experimental Biology 4(5): 46-52.

Atta, H.M. 2015. Biochemical studies on antibiotic production from Streptomyces sp.: Taxonomy, fermentation, isolation and biological properties. Journal of Saudi Chemical Society 19(1): 12-22.

Ballav, S., Kerkar, S., Thomas, S., Augustine, N. 2015. Halophilic and halotolerant actinomycetes from a marine saltern of Goa, India producing anti-bacterial metabolites. Journal of Bioscience and Bioengineering 119(3): 323-330.

Bettoli, V., Join-Lambert, O., Nassif, A. 2016. Antibiotic treatment of hidradenitis Suppurativa. Dermatologic Clinics 34(1): 81-89.

Chamberlain, K., Crawford, D.L. 1999. In vitro and in vivo antagonism of pathogenic turfgrass fungi by Streptomyces hygroscopicus strains YCED9 and WYE53. Journal of Industrial Microbiology and Biotechnology 23(1): 641-646.

Cheong, E.Y.L., Sandhu, A., Jayabalan, J., Le, T.T.K., Nhiep, N.T., Ho, H.T.M., Zwielehner, J., Bansal, N., Turner, M.S. 2014. Isolation of lactic acid bacteria with antifungal activity against the common cheese spoilage mould Penicillium commune and their potential as biopreservatives in cheese. Food Control 46; 91-97.

Chung, Y., Chien, C., Teng, K., Chou, S. 2006. Antioxidative and mutagenic properties of Zanthoxylumai lanthoides Sieb & zucc. Food Chemistry 97: 418-425.

Dalal, K., Munuganti, R., Morin, H., Lallous, N., Rennie, P.S., Cherkasov, A. 2016. Drug-Discovery Pipeline for Novel Inhibitors of the Androgen Receptor. Methods in Molecular Biology 1443:31-54.

Das, S., Ward, L.R., Burke, C. 2008. Prospects of using marine actinobacteria as probiotics in aquaculture. Applied Microbiology and Biotechology 81: 419-429.

El-Shatoury, S.A., El-Shenawy, N.S., Abd El-Salam, I.M. 2009. Antimicrobial, antitumor and in vivo cytotoxicity of actinomycetes inhabiting marine shellfish. World Journal of Microbiology and Biotechnology 25: 1547-1555.

Getha, K., Vikineswary, S. 2002. Antagonistic effects of Streptomyces violaceusniger strain G10 on Fusarium oxysporum f. sp. cubense race 4: indirect evidence for the role of antibiosis in the antagonistic process. Journal of Industrial Microbiology and Biotechnology 28: 303-310.

Grajeda-Iglesias, C., Salas, E., Barouh, N., Baréa, B., Panya, A., Figueroa-Espinoza, M.C. 2016. Antioxidant activity of protocatechuates evaluated by DPPH, ORAC, and CAT methods. Food Chemistry 194: 749-757

Han, Y., Li, L., Liu, J. 2013. Characterization of the airborne bacteria community at different distances from the rotating brushes in a wastewater treatment plant by 16S rRNA gene clone libraries. Journal of Environmental Sciences 25(1): 5-15.

Hassanien, M.F., Assiri, A.M., Alzohairy, A.M., Oraby, H.F. 2015. Health-promoting value and food applications of black cumin essential oil: an overview. Journal of Food Science and Technology 52(10):6136-6142.

Hathout, A.S., Abo-Sereih, N.A., Sabry, B.A., Sahab, A.F., Aly, S.E. 2015. Molecular identification and control of some pathogenic Fusarium species isolated from maize in Egypt. International Journal of ChemTech Research 7(1): 44-54.

Hathout, A.S., Aly, S.E. 2014. Biological detoxification of mycotoxins: a review. Annals of Microbiology 64(3): 905-919.

Iwasa M, Nakaya S, Maki Y, Marumoto S, Usami A, Miyazawa M. 2015. Identification of Aroma-active Compounds in Essential Oil from Uncaria Hook by Gas Chromatography- Mass Spectrometry and Gas Chromatography-Olfactometry. Journal of Oleo Science 64(8):825-833.

Janardhan, A., Kumar, A.P., Viswanath, B., Saigopal, D.V.R., Narasimha, G. 2014. Production of bioactive compounds by actinomycetes and their antioxidant properties. Biotechnology Research International Article ID 217030, 8 pages

Kekuda, T.R.P., Shobha, K.S., Onkarappa, R. 2010. Fascinating diversity and potent biological activities of actinomycetes metabolites. Journal of Pharmacy Research 3: 250-256.

Khamna, S., Yokota, A., Peberdy, J.F., Lumyong, S. 2009. Antifungal activity of Streptomyces spp. isolated from rhizosphere of Thai medicinal plants. International Journal of Integrative Biology 6(3): 143-147.

Kopf, A., Kostadinov, I., Wichels, A., Quast, C., Glöckner, F.O. 2015. Metatranscriptome of marine bacterioplankton during winter time in the North Sea assessed by total RNA sequencing. Marine Genomics 19: 45-46.

Krishnan, K., Mani, A., Jasmine, S. 2014. Cytotoxic activity of bioactive compound 1, 2- Benzene Dicarboxylic Acid, Mono 2- Ethyl hexyl Ester extracted from a marine derived Streptomyces sp. VITSJK8. International Journal of Molecular and Cellular Medicine 3(4): 246-254.

Kumar, P.P., Kumaravel, S., Lalitha, C. 2010. Screening of antioxidant activity, total phenolics and GC-MS study of vitexne-gundo. Africa Journal of Biochemistry Research 4(7): 191-195.

Lertcanawanichakul, M., Pondet, K., Kwantep, J. 2015. In vitro antimicrobial and antioxidant activities of bioactive compounds (secondary metabolites) extracted from Streptomyces lydicus A2. Journal of Applied Pharmaceutical Science 5: 17-21.

Lim, S.W., Kim, J.D., Kim, B.S., Hwang, B. 2000. Isolation and numerical identification of Streptomyces humidus strain S5-55 antagonistic to plant pathogenic Fungi. Plant Pathology Journal 16(4): 189-199.

Manivasagan P, Venkatesan J, Sivakumar K, Kim SK. 2014. Pharmaceutically active secondary metabolites of marine actinobacteria. Microbiological Research 169(4): 262-278.

Mechri, B., Manga, A.G.B., Tekaya, M., Attia, F., Cheheb, H., Ben Meriem, F., Braham, M., Boujnah, D., Hammami, M. 2014. Changes in microbial communities and carbohydrate profiles induced by the mycorrhizal fungus (Glomus intraradices) in rhizosphere of olive trees (Olea europaea L.). Applied Soil Ecology 75: 124-133

Mincer, T.J., Jensen, P.R., Kauffman, C.A., Fenical, W. 2002. Widespread and persistent populations of amajor new marine actinomycete taxon in ocean sediments. Applied Environmental Microbiology 68: 5005-5011.

Momose, I., Kawada, M. 2015. The therapeutic potential of microbial proteasome inhibitors. International Immunopharmacology S1567-5769(15)30183-1 doi: 10.1016/j. intimp.2015.11.013.

Mukherjee, G., Sen, S.K. 2006. Purification, characterization, and antifungal activity of chitinase from Streptomyces venezuelae P10. Current Microbiology 53: 265-269.

Mullowney, M.W., Hwang, C.H., Newsome, A.G., Wei, X., Tanouye, U., Wan, B., Carlson, S., Barranis, N.J., Ainmhire, E., Chen, W.L., Krishnamoorthy, K., White, J., Blair, R., Lee, H., Burdette, J.E., Rathod, P.K., Parish, T., Cho, S., Franzblau, S.G., Murphy, B.T. 2015. Diaza-anthracene antibiotics from a freshwater-derived actinomycete with selective antibacterial activity toward mycobacterium tuberculosis. ACS Infectious Diseases 1(4):168-174.

Myer, P.R., Kim, M., Freetly, H.C., Smith, T.P. 2016. Evaluation of 16S rRNA amplicon sequencing using two next-generation sequencing technologies for phylogenetic analysis of the rumen bacterial community in steers. Journal of Microbiological Methods 127:132-140.

Nandhini, S.U., Selvam, M.M. 2013. Bioactive compounds produced by Streptomyces strain. International Journal of Pharmacy and Pharmaceutical Sciences 5(1):176-178.

Nath, S., Chowdhury, S., Sarkar, S., Dora, K.C. 2013. Lactic Acid Bacteria-A potential Bio-preservative in sea food industry. Internatinal Journal of Advanced Research 1(6): 471-47.

Parrella, A., Lavorgna, M., Criscuolo, E., Russo, C., Isidori, N. 2014. Estrogenic activity and cytotoxicity of six anticancer drugs detected in water systems. Science of The Total Environment 485-486: 216-222.

Patel, J.D., Parmar, M., Patel, P., Rohit, P., Taviyad, R., Ansari, P., Singh, P.K. 2014. Dynamism of antimicrobial activity of actinomycetes a case study from undisturbed microbial niche. Advances in Microbiology 4: 324-334.

Piromyou, P., Noisangiam, R., Uchiyamia, H., Tittabutr, P., Boonkerd, N., Teaumroong, N. 2013. Indigenous Microbial Community Structure in Rhizosphere of Chinese Kale as Affected by Plant Growth-Promoting Rhizobacteria Inoculation. Pedosphere 23(5): 577-592

Ramazani, A., Moradi, S., Sorouri, R., Javani, S., Garshasbi, M. 2013. Screening for antibacterial activity of Streptomyces species isolated from Zanjan province Iran. International Journal of Pharmaceutical, Chemical and Biological Science 3(2): 342-349.

Rashad, F.M., Fathy, H.M., El-Zayat, A.S., Elghonaimy, A.M. 2015. Isolation and characterization of multifunctional Streptomyces species with antimicrobial, nematicidal and phytohormone activities from marine environments in Egypt. Microbiological Research 175:34-47.

Ravikumar, Y.S., Mahadevan, K.M., Kumaraswamy, M.N., Vaidya, V.P., Manjunatha, H., Kumar, V., Satyanarayana, N.D. 2008. Antioxidant, cytotoxic and genotoxic evaluation of alcoholic extract of Polyalthia cerasoides (Roxb.) Bedd. Environmental Toxicology and Pharmacology 26(2): 142-146.

Shaaban, M., Abdel-Razik, A.S., Abdel-Aziz, M.S., AbouZied, A.A., Fadel, M. 2013. Bioactive secondary metabolites from marine Streptomyces albogriseolus isolated from red sea coast. Journal of Applied Sciences Research 9(1): 996-1003.

Sineva, O.N., Terekhova, L.P. 2015. Selective Isolation of Rare Actinomycetes from Soil. Antibiot Khimioter 60(7-8):27-33.

Singh, S.P., Thumar, J.T., Gohel, S.D., Kikani, B., Shukla, B., Sharma, A., Dangar, K. 2013. Actinomycetes from marine habitats and their enzymatic potential. Marine Enzymes for Biocatalysis 2013: 191-214.

Sivakumar, K., Sahu, M.K., Thangaradjou, T., Kannan, L. 2007. Research on marine actinobacteria in India. Indian Journal of Microbiology 47(3):186-96.

Subashini, E, Kannabiran K. 2014. Isolation and identification of anti-ESBL (extended spectrum βlactamase) compound from marine streptomyces sp. VITSJK8. Journal Advances in Science Research 5: 13-18.

Subathra, D.C., Amrita, K., Nitin, J., Jemimah, N.S., Mohana, S.V. 2013. Screening of actinomycetes isolated from soil samples for antibacterial and antioxidant activity. International Journal of Pharmacy and Pharmaceutical Science 5(4): 483-489.

Subramani, R., Aalbersberg W. 2012. Marine actinomycetes: An ongoing source of novel bioactive metabolites. Microbiological Research 167(10): 571-580.

Sudha, S., Masilamani, S.M. 2012. Characterization of cytotoxic compound from marine sediment derived actinomycete Streptomyces avidinii strain SU4. Asian Pacific Journal of Tropical Biomedicine 2(10): 770-773.

Sui, Q., Liu, W., Lu, C., Liu, T., Qiu, J., Liu, X. 2009. Extraction and structural identification of the antifungal metabolite of Streptomyces lydicus A02. Sheng Wu Gong Cheng Xue Bao 25(6):840-846.

Taechowisan, T., Lu, C., Shen, Y., Lumyong, S. 2007. Antitumor activity of 4-arylcoumarins from endophytic Streptomyces aureofaciens CMUAc130. Journal of Cancer Research and Therapeutics 3(2):86-91.

Targowski T, Płusa T. 2015. Overall principles of treatment in case of toxicological threats. Polski Merkuriusz Lekarski 39(231):191-193.

Taylor, M.W., Radax, R., Steger, D., Wagner, M. 2007. Sponge-associated microorganisms: evolution, ecology, and biotechnological potential. Microbiology and Molecular Biology Reviews 71: 295-347.

Tenover, F.C. 2009. Antibiotic Susceptibility Testing. Encyclopedia of Microbiology (Third Edition), pp 67-77

Thirumalairaj, J., Shanmugasundaram, T., Sivasankari, K., Natarajaseenivasan, K., Balagurunathan, R. 2015. Isolation, screening and characterization of potent marine Streptomyces sp. PM105 against antibiotic resistant pathogens. Asian Journal of Pharmaceutical and Clinical Research 8(2): 439-443.

Wagner, C. 2015. Critical practicalities in sampling for mycotoxins in feed. Journal of AOAC International 98(2): 301-308.

Wang, S.L., Liang, T.W., Yen, Y.H. 2011. Bioconversion of chitin-containing wastes for the production of enzymes and bioactive materials. Carbohydrate Polymers 84(2): 732-742.

Wu, F., Munkvold, G.P. 2008. Mycotoxins in ethanol co-products: modeling economic impacts on the livestock industry and management strategies. Journal of Agricultural and Food Chemistry 56(11): 3900-3911.

Yip, W.K., Cheenpracha, S., Chang, L.C., Ho, C.C., Seow, H.F. 2010. Anti-proliferative and anti-invasive properties of a purified fraction from Streptomyces sp. H7372. International Journal of Oncology 37 (5): 1229-1241.

Published
17-09-2019
How to Cite
Abdel-Aziz, M., Hathout, A., El-Neleety, A., Hamed, A., Sabry, B., Aly, S., & Abdel-Wahhab, M. (2019). Molecular identification of actinomycetes with antimicrobial, antioxidant and anticancer properties. Comunicata Scientiae, 10(2), 218-231. https://doi.org/10.14295/cs.v10i2.2269
Section
Original Article