Relationship of Insecticidal Activity and Resistance to Proteolysis of Two Glucose/ Mannose Plant Lectins

Authors

  • Cleverson Diniz Freitas Universidade Federal do Piauí
  • Marcio Viana Ramos Universidade Federal do Piauí
  • Diego Pereira Souza Universidade Federal do Ceará
  • Jose Delano Barreto Marinho-Filho Universidade Federal do Ceará
  • Fabiano Moura Teixeira Universidade Federal do Ceará
  • Jefferson Soares Oliveira Universidade Feeral do Piauí

DOI:

https://doi.org/10.14295/cs.v2i1.15

Keywords:

Carbohydrate-binding proteins, Callosobruchus maculatus, Dysdercus peruvianus, lectins.

Abstract

Purified seed lectins from the legumes Canavalia brasiliensis (ConBr) and Cratylia floribunda (CFL)were tested for insecticidal activity against Callosobruchus maculatus (Coleoptera: Bruchidae)and Dysdercus peruvianus (Hemiptera: Pyrrhocoridae). ConBr and CFL caused negative effects onlarvae of C. maculatus at levels of 1% and 2% (w/w). Time of development of C. maculatus insects,grown in artificial seeds containing both lectins, was not significantly altered, but the emergencewas slightly diminished. Both lectins were resisted to proteolysis by gut proteases of C. maculatuslarvae. Artificial diets containing 1% and 2% of ConBr or CFL caused no effect on weight and survivalof D. peruvianus nymphs. Gut proteases of D. peruvianus were able to breakdown ConBr and CFLafter 2 hours of incubation. The ability of insects to digest the proteins was correlated directly withthe deleterious effect observed.

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Author Biographies

Cleverson Diniz Freitas, Universidade Federal do Piauí

Professor de Bioquimica da UFPI Campus Amilcar Ferreira Sobral-Floriano

Marcio Viana Ramos, Universidade Federal do Piauí

Departamento de Bioquimica da Universidade Federal do Ceará

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Published

2011-04-19

How to Cite

Freitas, C. D., Ramos, M. V., Souza, D. P., Marinho-Filho, J. D. B., Teixeira, F. M., & Oliveira, J. S. (2011). Relationship of Insecticidal Activity and Resistance to Proteolysis of Two Glucose/ Mannose Plant Lectins. Comunicata Scientiae, 2(1), 34–41. https://doi.org/10.14295/cs.v2i1.15

Issue

Vol. 2 No. 1 (2011)

Section

Original Article

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